doi: 10.1016/0014-5793(91)80340-9.
Actin depolymerization in the cyclic AMP-stimulated toad bladder epithelial cell, determined by the DNAse method
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- PMID: 1901549
- DOI: 10.1016/0014-5793(91)80340-9
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Actin depolymerization in the cyclic AMP-stimulated toad bladder epithelial cell, determined by the DNAse method
FEBS Lett.
.
Free article
Abstract
Previous studies with the rhodamine phalloidin binding assay have shown that antidiuretic hormone and 8-Br-cAMP rapidly depolymerize F-actin in toad bladder epithelial cells. We have extended these studies with the DNAse inhibition assay and have found that in isolated epithelial cell suspensions, G-actin increases from 37 to 56% of total actin following 8-br-cAMP stimulation. The G-actin concentration in the epithelial cell greatly exceeds its critical concentration, indicating the requirement for a G-actin sequestering protein or proteins in this system.
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