Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Mar 25;280(2):397-9.
doi: 10.1016/0014-5793(91)80340-9.

Actin depolymerization in the cyclic AMP-stimulated toad bladder epithelial cell, determined by the DNAse method

R M Hays  1 , U Lindberg
Affiliations
Free article

Actin depolymerization in the cyclic AMP-stimulated toad bladder epithelial cell, determined by the DNAse method

R M Hays et al. FEBS Lett. .
Free article

Abstract

Previous studies with the rhodamine phalloidin binding assay have shown that antidiuretic hormone and 8-Br-cAMP rapidly depolymerize F-actin in toad bladder epithelial cells. We have extended these studies with the DNAse inhibition assay and have found that in isolated epithelial cell suspensions, G-actin increases from 37 to 56% of total actin following 8-br-cAMP stimulation. The G-actin concentration in the epithelial cell greatly exceeds its critical concentration, indicating the requirement for a G-actin sequestering protein or proteins in this system.

PubMed Disclaimer

Publication types

LinkOut - more resources