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. 2008 Nov:Appendix 4:Appendix 4E.
doi: 10.1002/9780471729259.mca04es11.

Growth and maintenance of Vero cell lines

Nicole C Ammerman  1 , Magda Beier-Sexton, Abdu F Azad
Affiliations

Growth and maintenance of Vero cell lines

Nicole C Ammerman et al. Curr Protoc Microbiol. 2008 Nov.

Abstract

Vero cells are derived from the kidney of an African green monkey, and are one of the more commonly used mammalian continuous cell lines in microbiology and molecular and cell biology research. This unit includes protocols for the growth and maintenance of Vero cell lines in a research laboratory setting.

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Figures

Figure 1
Figure 1
Vero 76 cells (ATCC #CRL-1587), approximately 95% monolayer. These cells will need to be subcultured within one day.
See this image and copyright information in PMC

References

Literature Cited

    1. Chen SM, Popov VL, Feng HM, Wen J, Walker DH. Cultivation of Ehrlichia chaffeensis in mouse embryo, Vero, BGM, and L929 cells and study of Ehrlichia-induced cytopathic effect and plaque formation. Infect Immun. 1995;63:647–655. - PMC - PubMed
    1. Hegde R, Gomes AR, Byregowda SM, Hugar P, Giridhar P, Renukaprasad C. Standardization of large scale production of homologous live attenuated PPR vaccine in India. Trop Anim Health Prod. 2008;40:11–16. - PubMed
    1. Mariotti E, Mirabelli P, Di Noto R, Fortunato G, Salvatore F. Rapid detection of Mycoplasma in continuous cell lines using a selective biochemical test. Leukemia Research. 2008;32:323–326. - PubMed
    1. Nahapetian AT, Thomas JN, Thilly WG. Optimization of environment for high density Vero cell culture: effect of dissolved oxygen and nutrient supply on cell growth and changes in metabolites. J Cell Sci. 1986;81:65–103. - PubMed
    1. Phelan MC. Basic techniques in mammalian cell tissue culture. Curr Protoc Cell Biol. 2007;Chapter 1(Unit 1.1) - PubMed

Key References

    1. Coté RJ. Aseptic technique for cell culture. Curr Protoc Cell Biol. 2001;Chapter 1(Unit 1.3) This reference contains a protocol for proper sterile technique using a laminar flow hood.

    1. Simizu E, Terasima T, editors. Vero Cells – Origin, Properties and Biomedical Applications. Department of Microbiology, Chiba University; Chiba, Japan: 1988. This reference describes the history of the Vero cell line, and it also includes an English language translation of the original article describing Vero cells (Yasumura and Kawakita 1963, below).

    1. Yasumura Y, Kawakita Y. Studies on SV40 in tissue culture – preliminary step for cancer research “in vitro.”. Nihon Rinsho. 1963;21:1201–1215. article in Japanese. This is the original publication describing the isolation and initial propagation of the Vero cell line.

Internet Resources

    1. http://www.atcc.org The American Type Culture Collection (ATCC) website. The ATCC maintains a cell repository, which includes Vero cells. This site provides additional information regarding the growth and maintenance of Vero cells.

    1. http://www.ecacc.org.uk The European Collection of Animal Cell Cultures (ECACC) website. The ECACC maintains a cell repository, which includes Vero cells. This site provides additional information regarding the growth and maintenance of Vero cells.