The isolation and characterization of murine macrophages

Abstract

Macrophages are mononuclear phagocytes that are widely distributed throughout the body. These cells can contribute to development and homeostasis and participate in innate and adaptive immune responses. The physiology of macrophages can vary tremendously depending on the environment in which they reside and the local stimuli to which they are exposed. Macrophages are prodigious secretory cells, and in that role can promote and regulate immune responses and contribute to autoimmune pathologies. Macrophages are highly phagocytic, and in this capacity have long been considered to be essential immune effector cells. The important roles of macrophages in maintaining homeostasis and in contributing to tissue remodeling and wound healing is sometimes overlooked because of their vital role in host defense.

Figure 14.1.1

F4/80 and CD11b expression on bone marrow–derived macrophages. BALB/c macrophages were cultivated in L-cell conditioned medium for 7 days and then processed for flow cytometry. A 1:200 dilution of PE-conjugated anti-mouse F4/80 mAb BM8 (eBioscience, cat no. 12-4801) and a 1:800 dilution of PerCP Cy5.5 M1/70 antibody to CD11b (BD Pharmingen, cat no. 550993) were added. Cells were examined on a BD FACScalibur flow cytometer. For the color version of this figure go to http://www.currentprotocols.com.