Colonization of adventitious roots of Medicago truncatula by Pseudomonas fluorescens C7R12 as affected by arbuscular mycorrhiza

Abstract

Pseudomonas fluorescens C7R12 was previously shown to promote colonization of Medicago truncatula roots by Glomus mosseae BEG12. To gain more insight into the interaction between C7R12 and BEG12, the cell organization of C7R12 was characterized on adventitious roots mycorrhized or not with BEG12 and on extraradical hyphae. Bacterial cell observations were made using the immuno-fluorescence technique and confocal laser scanning microscopy. Five types of cell organization, so-called organization types (OT), were identified: small or large single cells, cells by pair and cells in microcolonies or in strings. The frequencies of each OT on the roots were expressed as the percentage of observations in which these OTs were represented. The OT frequencies on mycorrhizal and nonmycorrhizal roots differed significantly. Bacterial cells were more frequently single on mycorrhizal than on nonmycorrhizal roots, and in microcolonies and strings on nonmycorrhizal roots. Furthermore, the root area covered by bacterial cells, as assessed by image analysis, appeared to be significantly lower on mycorrhizal than on nonmycorrhizal roots. C7R12 cells were abundant on extraradical hyphae and organized both as single cells and microcolonies. Taken together, these results suggest that P. fluorescens C7R12 cells were less active and less abundant on mycorrhizal than on nonmycorrhizal roots.