Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Feb;57(2):606-9.
doi: 10.1128/aem.57.2.606-609.1991.

Species-specific detection of Listeria monocytogenes by DNA amplification

H G Deneer  1 , I Boychuk
Affiliations

Species-specific detection of Listeria monocytogenes by DNA amplification

H G Deneer et al. Appl Environ Microbiol. 1991 Feb.

Abstract

The polymerase chain reaction was used to detect and specifically identify Listeria monocytogenes. A 174-bp region of the listeriolysin O gene was shown to be specifically amplified in L. monocytogenes but not in other species of Listeria or in a number of other gram-positive and gram-negative organisms. Less than 50 organisms could routinely be detected by a procedure involving two rounds of 35 amplification cycles each and without the need for subsequent hybridization with labeled probes.

PubMed Disclaimer

References

    1. Appl Environ Microbiol. 1990 Sep;56(9):2930-2 - PubMed
    1. N Engl J Med. 1983 Jan 27;308(4):203-6 - PubMed
    1. J Bacteriol. 1987 Mar;169(3):1291-7 - PubMed
    1. Cold Spring Harb Symp Quant Biol. 1986;51 Pt 1:263-73 - PubMed
    1. Appl Environ Microbiol. 1987 Sep;53(9):2256-9 - PubMed

Publication types

MeSH terms

LinkOut - more resources