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. 1991 May;65(5):2702-6.
doi: 10.1128/JVI.65.5.2702-2706.1991.

Assembly of empty capsids by using baculovirus recombinants expressing human parvovirus B19 structural proteins

C S Brown  1 , J W Van Lent, J M Vlak, W J Spaan
Affiliations

Assembly of empty capsids by using baculovirus recombinants expressing human parvovirus B19 structural proteins

C S Brown et al. J Virol. 1991 May.

Abstract

Empty parvovirus B19 capsids were isolated from insect cells infected with a recombinant baculovirus expressing parvovirus B19 VP2 alone and also with a double-recombinant baculovirus expressing both VP1 and VP2. That VP2 alone can assemble to form capsids is a phenomenon not previously observed in parvoviruses. The stoichiometry of the capsids containing both VP1 and VP2 was similar to that previously observed in parvovirus B19-infected cells. The capsids were similar to native capsids in size and appearance, and their antigenicity was demonstrated by immunoprecipitation and enzyme-linked immunosorbent assay with B19-specific antibodies.

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