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. 2009 Feb 5;159(2):181-5.
doi: 10.1016/j.vetpar.2008.10.001. Epub 2008 Oct 11.

Is ITS-2 rDNA suitable marker for genetic characterization of Sarcoptes mites from different wild animals in different geographic areas?

S Alasaad  1 , D Soglia, V Spalenza, S Maione, R C Soriguer, J M Pérez, R Rasero, M P Ryser Degiorgis, H Nimmervoll, X Q Zhu, L Rossi
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Free article

Is ITS-2 rDNA suitable marker for genetic characterization of Sarcoptes mites from different wild animals in different geographic areas?

S Alasaad et al. Vet Parasitol. .
Free article

Abstract

The present study examined the relationship among individual Sarcoptes scabiei mites from 13 wild mammalian populations belonging to nine species in four European countries using the second internal transcribed spacer (ITS-2) of nuclear ribosomal DNA (rDNA) as genetic marker. The ITS-2 plus primer flanking 5.8S and 28S rDNA (ITS-2+) was amplified from individual mites by polymerase chain reaction (PCR) and the amplicons were sequenced directly. A total of 148 ITS-2+ sequences of 404bp in length were obtained and 67 variable sites were identified (16.59%). UPGMA analyses did not show any geographical or host-specific clustering, and a similar outcome was obtained using population pairwise Fst statistics. These results demonstrated that ITS-2 rDNA does not appear to be suitable for examining genetic diversity among mite populations.

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