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. 2008 Jan;145(1):108-13.
doi: 10.1007/s10517-008-0034-5.

Detection of mutations in Mycobacterium tuberculosis genome determining resistance to fluoroquinolones by hybridization on biological microchips

O V Antonova  1 , D A Gryadunov, S A Lapa, A V Kuz'min, E E Larionova, T G Smirnova, E Y Nosova, O I Skotnikova, L N Chernousova, A M Moroz, A S Zasedatelev, V M Mikhailovich
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Detection of mutations in Mycobacterium tuberculosis genome determining resistance to fluoroquinolones by hybridization on biological microchips

O V Antonova et al. Bull Exp Biol Med. 2008 Jan.

Abstract

We developed a method of identification of Mycobacterium tuberculosis with simultaneous evaluation of the sensitivity to fluoroquinolones on a biological microchip array. The method of multiplex two-staged PCR followed by hybridization of a biochip makes it possible to detect 8 mutant variants of gyrA gene occurring in fluoroquinolone-resistant strains (approximately 85% all resistant forms) within 1 day. Using this method we analyzed 107 cultures isolated from patients with tuberculosis and 78 sputum samples. Mutations in gyrA gene were detected in 48 (92%) resistant strains. Natural S95T polymorphism in gyrA gene was detected in all resistant and in 76% sensitive strains. The sensitivity and specificity of the proposed method calculated on the basis of the analysis of sputum samples (n=78) were 94 and 100%, respectively.

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