Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Case Reports
. 1991 Mar;82(3):240-4.
doi: 10.1111/j.1349-7006.1991.tb01835.x.

Verification of HTLV-I infection in the Solomon Islands by virus isolation and gene amplification

R Yanagihara  1 , A B Ajdukiewicz, V R Nerurkar, R M Garruto, D C Gajdusek
Affiliations
Case Reports

Verification of HTLV-I infection in the Solomon Islands by virus isolation and gene amplification

R Yanagihara et al. Jpn J Cancer Res. 1991 Mar.

Abstract

We report the detection of human T-lymphotropic virus type I (HTLV-I) genomic sequences by polymerase chain reaction in lymphocyte cultures of three unrelated native Solomon Islanders, including a patient with HTLV-I myeloneuropathy, residing in widely separated regions. In addition, we have isolated HTLV-I from T-cell lines derived from two of these individuals. Virus-specific proteins of 15, 19, 24, 46 and 53 kilodaltons were detected by immunofluorescence and Western immunoblot, using serum from a Colombian patient with HTLV-I myeloneuropathy, sera from HTLV-I-infected rabbits, and monoclonal and polyclonal antibodies against HTLV-I gag and env gene products. Amplification of HTLV-I gag, pol and env sequences by polymerase chain reaction confirmed that the viral isolates were HTLV-I, not HTLV-II. Our data clearly demonstrate that HTLV-I does exist in Melanesia. Although the Solomon Islands viral isolates resemble prototype strains of HTLV-I, we believe they represent variants of HTLV-I, particularly in the light of our recent isolation of an HTLV-I variant from Papua New Guinea. Nucleotide sequence analysis of these viral strains, now in progress, should clarify the molecular epidemiology and phylogeny of HTLV-I.

PubMed Disclaimer

References

    1. ) Goudsmit , J. , Asher , D. M , Garruto , R. M. , Mora , C. , Jenkins , C. , Pomeroy , K. L. , Askins , H. and Gajdusek , D. C.Antibodies to HTLV‐I in populations of the Western Pacific . Abstr. 16th Pac. Sci. Congr. , 73 ( 1987. ).
    1. ) Kazura , J. W. , Saxinger , W. C. , Wenger , J. , Forsyth , K. , Lederman , M. M. , Gillespie , J. A. , Carpenter , C. J. and Alpers , M. P.Epidemiology of human T‐cell leukemia virus type I infection in East Sepik Province, Papua New Guinea . J. Infect. Dis. , 155 , 1100 – 1107 ( 1987. ). - PubMed
    1. ) Asher , D. M. , Goudsmit , J. , Pomeroy , K. L. , Garruto , R. M. , Bakker , M. , Ono , S. G. , Elliott , R , Harris , K. , Askins , H. , Eldadah , Z. , Goldstein , A. D. and Gajdusek , D. C.Antibodies to HTLV‐I in populations of the southwestern Pacific . J. Med. Virol , 26 , 339 – 351 ( 1988. ). - PubMed
    1. ) Brindle , R. J. , Eglin , R. P. , Parsons , A. J. , Hill , A. V. S. and Selkon , J. B.HTLV‐I, HIV‐I, hepatitis B and hepatitis delta in the Pacific and southeast Asia: a serological survey . Epidemiol Infect. , 100 , 153 – 156 ( 1988. ). - PMC - PubMed
    1. ) Babona , D. V. and Nurse , G. T.HTLV‐I antibodies in Papua New Guinea . Lancet , ii , 1148 ( 1988. ). - PubMed

Publication types

Substances

LinkOut - more resources