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. 2009 Feb;163(2):123-6.
doi: 10.1016/j.molbiopara.2008.10.004. Epub 2008 Nov 5.

A green fluorescent protein-based assay for determining gametocyte production in Plasmodium falciparum

Matthew W A Dixon  1 , Christopher L Peatey, Donald L Gardiner, Katharine R Trenholme
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A green fluorescent protein-based assay for determining gametocyte production in Plasmodium falciparum

Matthew W A Dixon et al. Mol Biochem Parasitol. 2009 Feb.

Abstract

Study of the formation of the sexual blood stages of the malaria parasite has been significantly hampered by the absence of a reliable, reproducible assay devoid of operator bias and error. Here we report on the development of an assay utilizing a green fluorescent protein chimera of the early sexual blood stage protein Pfs16 as a marker for commitment to gametocytogenesis. Analysis of parasites via fluorescence activated cell sorting allows for the accurate assessment of gametocyte production well before morphological changes are apparent.

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