Ovarian function of the trout preovulatory ovary: new insights from recent gene expression studies

Abstract

During the preovulatory period the follicle-enclosed oocyte progressively acquires maturational and developmental competence. In addition, the follicle is also preparing for the release of the oocyte from the follicle at ovulation. Using real-time PCR and cDNA microarrays we have investigated the molecular mechanisms of oocyte competence acquisition and ovulation in rainbow trout (Oncorhynchus mykiss) by monitoring gene expression in the preovulatory ovary. These studies have demonstrated that many molecular events related to maturational competence and developmental competence acquisition, and ovulation occur concomitantly in the preovulatory ovarian follicle. Oocyte maturational competence acquisition is associated with a decrease of estrogen synthesis and signaling capacities. We also observed a differential expression of genes encoding for igfs and related binding protein, members of the TGF beta superfamily, proteins involved in ion and water transport, bone morphogenetic proteins, and cathepsins. In addition, our observation of a strong up-regulation, prior to ovulation, of genes encoding for proteins putatively involved in proteolysis, inflammation, coagulation, vasodilatation, and angiogenesis further supports the hypothesis comparing ovulation with an inflammatory-like reaction. Together, our results suggest that a finely tuned cross-talk exists between oocyte and follicular layers and between the ovulatory process and the oocyte maturational and developmental competence acquisition processes.