Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Feb;53(2):818-20.
doi: 10.1128/AAC.00987-08. Epub 2008 Nov 24.

First report on a hyperepidemic clone of KPC-3-producing Klebsiella pneumoniae in Israel genetically related to a strain causing outbreaks in the United States

Shiri Navon-Venezia  1 , Azita Leavitt, Mitchell J Schwaber, J Kamile Rasheed, Arjun Srinivasan, Jean B Patel, Yehuda Carmeli; Israeli KPC Kpn Study Group
Collaborators, Affiliations

First report on a hyperepidemic clone of KPC-3-producing Klebsiella pneumoniae in Israel genetically related to a strain causing outbreaks in the United States

Shiri Navon-Venezia et al. Antimicrob Agents Chemother. 2009 Feb.

Abstract

A highly epidemic carbapenem-resistant clone of KPC-3-producing Klebsiella pneumoniae emerged in Israel in 2006, causing a nationwide outbreak. This clone was genetically related to outbreak strains from the United States isolated in 2000 but differed in KPC-carrying plasmids. The threat of the global spread of hyperepidemic, extensively drug-resistant bacterial strains should be recognized and confronted.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
(A) PFGE (SpeI restricted) of 14 representative KPC-3-producing, carbapenem-resistant K. pneumoniae isolates from various Israeli hospitals (lanes 1 to 14) demonstrates a nationwide epidemic clone different from a KPC-2-producing clone reported previously in our hospital (6) (lane 15). (B) Genetic relatedness of the predominant Israeli epidemic clone (lane 10) with nine KPC-3-producing K. pneumoniae isolates involved in outbreaks in the United States, from New Jersey (NJ; lanes 1 and 2), Arizona (AZ; lanes 3 to 5), and New York (NY; lanes 6 to 9). M, lambda ladder (New England Biolabs).
FIG. 2.
FIG. 2.
A comparison of blaKPC-3-encoding plasmids (A) and their EcoRI restriction patterns (B) from five Israeli K. pneumoniae isolates (lanes 1 to 5 in each panel) and five of the nine genetically related K. pneumoniae U.S. isolates (lanes 6 to 10 in each panel). Analysis was performed on plasmids isolated from E. coli GeneHogs transformants carrying the blaKPC-3-encoding plasmids. Lanes 1 to 5, representative Israeli isolates from 2006 to 2008; lanes 6 to 10, representative U.S. isolates from 2000 and 2006; lanes 6 and 7, two isolates from a New York medical center; lanes 8 to 10, one isolate from New Jersey and two isolates from Arizona. For panel A, lane M is a BAC-Tracker supercoiled DNA ladder (Epicentre Biotechnologies); for panel B, lane M is a GeneRuler 1-kb DNA ladder (Fermentas Life Sciences).
See this image and copyright information in PMC

References

    1. Bradford, P. A., S. Bratu, C. Urban, M. Visalli, N. Mariano, D. Landman, J. J. Rahal, S. Brooks, S. Cebular, and J. Quale. 2004. Emergence of carbapenem-resistant Klebsiella species possessing the class A carbapenem-hydrolyzing KPC-2 and inhibitor-resistant TEM-30 β-lactamases in New York City. Clin. Infect. Dis. 39:55-60. - PubMed
    1. Bratu, S., D. Landman, R. Haag, R. Rocco, A. Eramo, M. Alam, and J. Quale. 2005. Rapid spread of carbapenem-resistant Klebsiella pneumoniae in New York City. Arch. Intern. Med. 165:1430-1435. - PubMed
    1. Clinical and Laboratory Standards Institute/NCCLS. 2005. Performance standards for antimicrobial susceptibility testing; 15th informational supplement. CLSI/NCCLS M100-S15. Clinical and Laboratory Standards Institute, Wayne, PA.
    1. Desphande, L. M., R. N. Jones, T. R. Fritsche, and H. S. Sader. 2006. Occurrence and characterization of carbapenemase-producing Enterobacteriaceae: report from the SENTRY Antimicrobial Surveillance Program (2000-2004). Microb. Drug Resist. 12:223-230. - PubMed
    1. Kazakova, S. V., J. C. Hageman, M. Matava, A. Srinivasan, L. Phelan, B. Garfinkel, T. Boo, S. McAllister, J. Anderson, B. Jensen, D. Dodson, D. Lonsway, L. K. McDougal, M. Arduino, V. J. Fraser, G. Killgore, F. C. Tenover, S. Cody, and D. B. Jernigan. 2005. A clone of methicillin-resistant Staphylococcus aureus among professional football players. N. Engl. J. Med. 352:468-475. - PubMed

Publication types

MeSH terms