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. 1991 Jun;173(11):3414-8.
doi: 10.1128/jb.173.11.3414-3418.1991.

Isolation of a coenzyme M-auxotrophic mutant and transformation by electroporation in Methanococcus voltae

P A Micheletti  1 , K A Sment, J Konisky
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Isolation of a coenzyme M-auxotrophic mutant and transformation by electroporation in Methanococcus voltae

P A Micheletti et al. J Bacteriol. 1991 Jun.

Abstract

An auxotrophic mutant of Methanococcus voltae was isolated that required coenzyme M (CoM) for growth. With the mutant as a recipient, conditions were developed that allowed the introduction of wild-type CoM+ DNA into the mutant methanogen via electroporation. This method also allowed the rescue of both a histidine and purine auxotroph as well as the introduction of DNA determining resistance to the CoM analog 2-bromoethanesulfonic acid. Electroporation of the CoM(+)-determining DNA was 50- to 80-fold more efficient than natural transformation.

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References

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