Regional expression of NAD(P)H oxidase and superoxide dismutase in the brain of rats with neurogenic hypertension

Abstract

Background: Single injection of small quantities of phenol into the kidney cortex causes hypertension which is mediated by renal afferent sympathetic pathway activation. This phenomenon can be prevented by superoxide dismutase (SOD) infusion in the lateral ventricle, suggesting the role of superoxide (O(2)(-).) in noradrenergic control of arterial pressure. Since NAD(P)H oxidase is a major source of O(2)(-)., we tested the hypothesis that hypertension in this model may be associated with upregulation of NAD(P)H oxidase in relevant regions of brain.

Methods: NAD(P)H oxidase subunits, mitochondrial (MnSOD) and cytoplasmic (CuZnSOD) SOD were measured in rats 4 weeks after injection of phenol or saline in the left kidney cortex.

Results: Phenol-injected rats exhibited hypertension, upregulation of gp91(phox), p22(phox), p47(phox) and p67(phox) in the medulla, gp91(phox) and p22(phox) in pons and gp91(phox) in hypothalamus. This was associated with upregulation of MnSOD with little change in CuZnSOD.

Conclusions: Chronic hypertension in phenol-injected rats is associated with upregulation of NAD(P)H oxidase and hence increased O(2)(-). production capacity in the key regions of the brain involved in regulation of blood pressure. Since reactive oxygen species can intensify central noradrenergic activity, the observed maladaptive changes may contribute to the genesis and maintenance of the associated hypertension.

Fig. 1.

Systolic arterial pressure and serum creatinine concentration in rats obtained at baseline and 4 weeks after injection of saline or phenol in the renal cortex. n = 6 rats in each group; * p < 0.05.

Fig. 2.

Protein abundance of gp91^phox, p22^phox, p47^phox, p67^phox in the medulla of rats obtained at baseline and 4 weeks after injection of saline or phenol in the renal cortex. n = 6 rats in each group; * p < 0.05.

Fig. 3.

Protein abundance of gp91^phox, p22^phox, p47^phox, p67^phox in the pons of rats obtained at baseline and 4 weeks after injection of saline or phenol in the renal cortex. n = 6 rats in each group; * p < 0.05.

Fig. 4.

Protein abundance of gp91^phox, p22^phox, p47^phox, p67^phox in the hypothalamus of rats obtained at baseline and 4 weeks after injection of saline or phenol in the renal cortex. n = 6 rats in each group; * p < 0.05.

Fig. 5.

Protein abundance of MnSOD and CuZnSOD in the medulla of rats obtained at baseline and 4 weeks after injection of saline or phenol in the renal cortex. n = 6 rats in each group; * p < 0.05.

Fig. 6.

Protein abundance of MnSOD and CuZnSOD in the pons of rats obtained at baseline and 4 weeks after injection of saline or phenol in the renal cortex. n = 6 rats in each group; * p < 0.05.

Fig. 7.

Protein abundance of MnSOD and CuZnSOD in the hypothalamus of rats obtained at baseline and 4 weeks after injection of saline or phenol in the renal cortex. n = 6 rats in each group; * p < 0.05.