doi: 10.1016/0006-8993(91)90532-z.
Neurobiochemical evidence for calcium-induced depolarization of EGTA-pretreated hippocampal synaptosomes
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- PMID: 1905174
- DOI: 10.1016/0006-8993(91)90532-z
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Neurobiochemical evidence for calcium-induced depolarization of EGTA-pretreated hippocampal synaptosomes
Brain Res.
.
Abstract
The neurochemical effects of calcium were determined in hippocampal cholinergic synaptosomes which had been prepared and preincubated in calcium-free medium containing 50 microM EGTA. Calcium (and barium) reversibly stimulated [3H]acetylcholine release and produced a long-lasting elevation of high-affinity [3H]choline uptake. Both effects were blocked by omega-conotoxin and substantially reduced by tetrodotoxin. Together, these data indicate that calcium causes membrane depolarization and is associated with opening of voltage-gated sodium channels in EGTA-pretreated synaptosomes.
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