doi: 10.1021/bi00635a012.
Partial purification and characterization of an N2-guanine RNA methyltransferase from chicken embryos
- PMID: 19056
- DOI: 10.1021/bi00635a012
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Partial purification and characterization of an N2-guanine RNA methyltransferase from chicken embryos
Biochemistry.
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Abstract
An N2-guanine RNA methyltransferase has been purified 1000-fold from chick embryo homogenates by phosphocellulose chromatography followed by chromatography on S-adenosylhomocystein-Sepharose. The enzyme was shown to methylate the G10 position of Escherichia coli B tRNAPhe and has a Km of 3X10(-7) M for tRNAPhe and 1.38 X 10(-6) M for S-adenosylmethionine. The molecular weight was estimated to be 77 000 by gel filtration and the pH optimum was 8.0 to 8.5. Magnesium ion was not required for activity but it stimulated the rate of methylation 1.5-fold with an optimum at 12 mM. Ammonium ion stimulated activity about twofold with an optimum at about 83 mM. Sodium and potassium ions above 0.1 M were inhibitory.
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