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. 1977 Feb;4(2):353-71.
doi: 10.1093/nar/4.2.353.

Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides

J Stawinski, T Hozumi, S A Narang, C P Bahl, R Wu
Free PMC article

Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides

J Stawinski et al. Nucleic Acids Res. 1977 Feb.
Free PMC article

Abstract

The modified triester approach has been further improved and refined to the synthesis of defined sequences of deoxyribo-oligonucleotides. Improvements include arylsulfonyltetrazoles as faster and milder condensing agents, benzenesulfonic acid to avoid depurination during deblocking of trityl protecting groups and improved chromatographic procedures for purification of triester intermediates and purification of the final product containing 3'-5' phosphodiester linkages.

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References

    1. Nucleic Acids Res. 1974 Mar;1(3):331-53 - PubMed
    1. J Mol Biol. 1975 Feb 25;92(2):331-9 - PubMed
    1. J Mol Biol. 1975 Sep 5;97(1):123-5 - PubMed
    1. J Am Chem Soc. 1975 Dec 10;97(25):7327-32 - PubMed
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