Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Feb;4(2):373-80.
doi: 10.1093/nar/4.2.373.

Phosphorylation of tRNA by T4 polynucleotide kinase

J R Lillehaug, K Kleppe
Free PMC article

Phosphorylation of tRNA by T4 polynucleotide kinase

J R Lillehaug et al. Nucleic Acids Res. 1977 Feb.
Free PMC article

Abstract

The phosphorylation of various intact tRNA species by T4 polynucleotide kinase has been studied. The apparent Michaelis constant was on the average found to be 100 times lower than for some single-stranded DNAs previously studied. (J.R. Lillehaug and K. Kleppe, (1975) Biochemistry, 14, 1221). Conditions which result in complete phosphorylation of different tRNA species have also been established. Studies on equilibrium constants and the reversibility of the reaction revealed that the phosphorylation reaction is not a true equilibrium reaction under the conditions used in this work.

PubMed Disclaimer

References

    1. Biochem J. 1964 Jan;90(1):147-9 - PubMed
    1. Science. 1976 May 28;192(4242):853-8 - PubMed
    1. Biochim Biophys Acta. 1975 Dec 4;414(2):93-8 - PubMed
    1. Biochemistry. 1975 Mar 25;14(6):1225-9 - PubMed
    1. Biochem Biophys Res Commun. 1975 Oct 6;66(3):962-9 - PubMed