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Comment
. 2008 Dec 23;105(51):E119; author reply E120.
doi: 10.1073/pnas.0810077106. Epub 2008 Dec 5.

Macromolecular crowding in vitro as means of emulating cellular interiors: when less might be more

K S Harve, R Vigneshwar, R Rajagopalan, M Raghunath
Comment

Macromolecular crowding in vitro as means of emulating cellular interiors: when less might be more

K S Harve et al. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1.
Fig. 1.
Size and structural changes in Fc70. (A) Decrease of hydrodynamic radius with increasing concentrations of Fc70 in 20 mM phosphate buffer. (B) CD spectra demonstrate shift of the maximum absorbance to the right with increasing Fc concentrations.
Fig. 2.
Fig. 2.
Urea-induced effects on Fc70. (A) Large increase in polydispersity of Fc70 molecules in 0.9 M urea/20 mM phosphate buffer deduced from DLS. (B) CD spectra indicate significant effects of urea on Fc70 structure.
See this image and copyright information in PMC

Comment on

References

    1. Homouz D, Perham M, Samiotakis A, Cheung MS, Wittung-Stafshede P. Crowded, cell-like environment induces shape changes in aspherical protein. Proc Natl Acad Sci USA. 2008;105:11754–11759. - PMC - PubMed
    1. Harve KS, Lareu RR, Rajagopalan R, Raghunath M. Macromolecular crowding in biological systems: Dynamic light scattering (DLS) to quantify the excluded volume effect. Biophys Rev Lett. 2006;1:317–325.
    1. Lareu RR, Harve KS, Raghunath M. Emulating a crowded intracellular environment in vitro dramatically improves RT-PCR performance. Biochem Biophys Res Commun. 2007;363:171–177. - PubMed
    1. Ellis RJ. Macromolecular crowding: Obvious but under-appreciated. Trends Biochem Sci. 2001;26:597–604. - PubMed

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