[From nucleic acids to proteins]
- PMID: 1906104
- DOI: 10.1248/yakushi1947.111.3_170
[From nucleic acids to proteins]
Abstract
Since elucidation of structure of deoxyribonucleic acid (DNA) as genetic material, it was established that DNA makes ribonucleic acid, which makes protein according to the original information given in DNA base as base sequences. From these basic idea of genetic flow system there was emerged the word "protein engineering". According to this principle, engineering of h-lysozyme, RNase H and their relevant proteins was described. A triosephosphate isomerase barrel type protein was designed and expressed.
Similar articles
-
[Structures and functions of ribonucleases].Yakugaku Zasshi. 1997 Sep;117(9):561-82. doi: 10.1248/yakushi1947.117.9_561. Yakugaku Zasshi. 1997. PMID: 9357326 Review. Japanese.
-
Insertion in barnase of a loop sequence from ribonuclease T1. Investigating sequence and structure alignments by protein engineering.Eur J Biochem. 1994 May 1;221(3):1003-12. doi: 10.1111/j.1432-1033.1994.tb18817.x. Eur J Biochem. 1994. PMID: 8181455
-
Improving purification of recombinant ribonuclease T1.J Biotechnol. 1992 Jun;24(2):189-94. doi: 10.1016/0168-1656(92)90122-p. J Biotechnol. 1992. PMID: 1368355
-
Cloning and nucleotide sequence of the ribonuclease T1 gene (rntA) from Aspergillus oryzae and its expression in Saccharomyces cerevisiae and Aspergillus oryzae.Biosci Biotechnol Biochem. 1995 Oct;59(10):1869-74. doi: 10.1271/bbb.59.1869. Biosci Biotechnol Biochem. 1995. PMID: 8534978
-
The ribonuclease T1 family.Methods Enzymol. 2001;341:28-41. doi: 10.1016/s0076-6879(01)41143-8. Methods Enzymol. 2001. PMID: 11582784 Review. No abstract available.