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. 1977 Jan;22(1):83-5.

In vitro stimulation of bovine circulating lymphocytes by parainfluenza type 3 virus

K Johnson, B Morein
  • PMID: 190660

In vitro stimulation of bovine circulating lymphocytes by parainfluenza type 3 virus

K Johnson et al. Res Vet Sci. 1977 Jan.

Abstract

Bovine circulating lymphocytes from animals sero-positive to parainfluenza-3 virus (PIV-3) could be stimulated by PIV-3 antigen in a microculture system. A semiautomatic multiple sample harvester was used to collect the lymphocytes from the microplates. Ultraviolet-killed PIV-3 induced better stimulation values than formalin or heat-treated virus. The highest stimulation values were obtained after a stimulation time of three to five days.

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