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. 2009:501:69-76.
doi: 10.1007/978-1-60327-164-6_7.

Enumeration of bacteriophages by double agar overlay plaque assay

Andrew M Kropinski  1 , Amanda Mazzocco, Thomas E Waddell, Erika Lingohr, Roger P Johnson
Affiliations

Enumeration of bacteriophages by double agar overlay plaque assay

Andrew M Kropinski et al. Methods Mol Biol. 2009.

Abstract

The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In this chapter the classical overlay protocol is described.

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