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. 2009:501:113-26.
doi: 10.1007/978-1-60327-164-6_12.

Basic phage electron microscopy

Hans-W Ackermann  1
Affiliations

Basic phage electron microscopy

Hans-W Ackermann. Methods Mol Biol. 2009.

Abstract

Negative staining of purified viruses is the most important electron microscopical technique in virology. The principal stains are phosphotungstate and uranyl acetate, both of which have problems and advantages. Particular problems are encountered in photography, calibration of magnification, measurements, and interpretation of artifacts.

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