Aldosterone and chloride conductance of amphibian skin

Abstract

Chloride influx (JCl) across the skin of toads maintained in dilute MgCl2 or Na2SO4 was determined after overnight incubation with(out) aldosterone, and related to mitochondria-rich cell (MRC) density of the preparations. Adaptation to MgCl2 vs. Na2SO4 was reflected by higher plasma aldosterone in the former group (17 vs. 3 nmol/l, respectively) while JCl was lower, even after overnight incubation (172 vs. 318 pmol cm-2 s-1). Incubation with aldosterone induced a more pronounced increase in JCl in the case of Na2SO4- vs. MgCl2-adapted toads (delta JCl: 242 vs. 25 pmol cm-2 s-1, respectively), which could be related to difference in MRC density between these two groups (1078 vs. 615 cells/mm2, respectively). On the other hand, the in vitro effect of aldosterone on Na+ transport (assessed by Isc) was equally pronounced in both groups, and thus independent of MRC density. These data suggest that aldosterone, rather than being involved in MRC proliferation, stimulates Cl- conductance by influencing the functional state of MRC.