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Review
. 2009 Apr;48(4):389-99.
doi: 10.1002/mc.20501.

Repair of DNA lesions associated with triplex-forming oligonucleotides

Joanna Y Chin  1 , Peter M Glazer
Affiliations
Review

Repair of DNA lesions associated with triplex-forming oligonucleotides

Joanna Y Chin et al. Mol Carcinog. 2009 Apr.

Abstract

Triplex-forming oligonucleotides (TFOs) are gene targeting tools that can bind in the major groove of duplex DNA in a sequence-specific manner. When bound to DNA, TFOs can inhibit gene expression, can position DNA-reactive agents to specific locations in the genome, or can induce targeted mutagenesis and recombination. There is evidence that third strand binding, alone or with an associated cross-link, is recognized and metabolized by DNA repair factors, particularly the nucleotide excision repair pathway. This review examines the evidence for DNA repair of triplex-associated lesions.

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Figures

Figure 1
Figure 1
Triplex-forming oligonucleotides can mediate various biological activities upon binding to duplex DNA. Reprinted with permission from [58].
Figure 2
Figure 2
TFOs bind to polypurine sequences in DNA via Hoogsteen base-pairing. Pyrimidine and purine TFOs differ in third strand base composition and third strand orientation with respect to the polypurine strand of DNA. Reprinted with permission from [58].
See this image and copyright information in PMC

References

    1. Felsenfeld G, Davies DR, Rich A. Formation of a three-stranded polynucleotide molecule. J Am Chem Soc. 1957;79:2023–2024.
    1. Moser HE, Dervan PB. Sequence-specific cleavage of double helical DNA by triple helix formation. Science. 1987;238(4827):645–650. - PubMed
    1. Praseuth D, Perrouault L, Le Doan T, Chassignol M, Thuong N, Helene C. Sequence-specific binding and photocrosslinking of alpha and beta oligodeoxynucleotides to the major groove of DNA via triple-helix formation. Proc Natl Acad Sci U S A. 1988;85(5):1349–1353. - PMC - PubMed
    1. Letai AG, Palladino MA, Fromm E, Rizzo V, Fresco JR. Specificity in formation of triple-stranded nucleic acid helical complexes: studies with agarose-linked polyribonucleotide affinity columns. Biochemistry. 1988;27(26):9108–9112. - PubMed
    1. Cooney M, Czernuszewicz G, Postel EH, Flint SJ, Hogan ME. Site-specific oligonucleotide binding represses transcription of the human c-myc gene in vitro. Science. 1988;241(4864):456–459. - PubMed

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