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Review
. 2009 Apr;47(4):877-84.
doi: 10.1128/JCM.01685-08. Epub 2008 Dec 10.

Sequence-based identification of Aspergillus, fusarium, and mucorales species in the clinical mycology laboratory: where are we and where should we go from here?

S A Balajee  1 , A M Borman, M E Brandt, J Cano, M Cuenca-Estrella, E Dannaoui, J Guarro, G Haase, C C Kibbler, W Meyer, K O'Donnell, C A Petti, J L Rodriguez-Tudela, D Sutton, A Velegraki, B L Wickes
Affiliations
Review

Sequence-based identification of Aspergillus, fusarium, and mucorales species in the clinical mycology laboratory: where are we and where should we go from here?

S A Balajee et al. J Clin Microbiol. 2009 Apr.
No abstract available

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Figures

FIG. 1.
FIG. 1.
RNA polymerase II second largest subunit (RPB2) phylogeny of Fusarium (modified from Fig. 1 in reference 30), showing the utility of DNA sequence data from various loci for resolving at or near the species level within six medically important species complexes. GFSC, Gibberella fujikuroi species complex; FOSC, Fusarium oxysporum species complex; FIESC, Fusarium incarnatum-equiseti species complex; FCSC, Fusarium chlamydosporum species complex; FSSC, Fusarium solani species complex; FDSC, Fusarium dimerum species complex. Loci include EF-1α (translation elongation factor), β-TUB (β-tubulin), and CAM (calmodulin). Numbers above the internodes represent the frequency (%) with which they were recovered from 1,000 bootstrap replicates of the data. A sequence of Lecanicillium lecanii was used to root the phylogeny.
FIG. 2.
FIG. 2.
An algorithm for identification of an unknown filamentous fungal species in a clinical microbiology laboratory. *, many factors affect percent identity scores including quality and length of query sequence and the number and accuracy of existing GenBank records for same species and locus. +, identification to species level within the Aspergillus and Fusarium complex can be achieved by comparative sequence analyses of protein coding regions.
See this image and copyright information in PMC

References

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