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Comparative Study
. 2009 Feb;47(2):441-4.
doi: 10.1128/JCM.01989-08. Epub 2008 Dec 10.

Comparison of commercial and in-house real-time PCR assays used for detection of Mycoplasma pneumoniae

Roger Dumke  1 , Enno Jacobs
Affiliations
Comparative Study

Comparison of commercial and in-house real-time PCR assays used for detection of Mycoplasma pneumoniae

Roger Dumke et al. J Clin Microbiol. 2009 Feb.

Abstract

We tested two commercial and three in-house PCR assays under standardized conditions to detect Mycoplasma pneumoniae. All five procedures were able to demonstrate M. pneumoniae DNA in a concentration comparable to 1 CFU/microl, but the mean crossing points resulted in differences in the concentration of the genome copies of a factor of 20.

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References

    1. Apfalter, P., U. Reischl, and R. M. Hammerschlag. 2005. In-house nucleic acid amplification assays in research: how much quality control is needed before one can rely upon the results? J. Clin. Microbiol. 435835-5841. - PMC - PubMed
    1. Atkinson, T. P., M. F. Balish, and K. B. Waites. 2008. Epidemiology, clinical manifestations, pathogenesis and laboratory detection of Mycoplasma pneumoniae infections. FEMS Microbiol. Rev. 32956-973. - PubMed
    1. Daxboeck, F., G. Khanakah, C. Bauer, M. Stadler, H. Hofmann, and G. Stanek. 2005. Detection of Mycoplasma pneumoniae in serum specimens from patients with mycoplasma pneumonia by PCR. Int. J. Med. Microbiol. 295279-285. - PubMed
    1. Dorigo-Zetsma, J. W., B. Wilbrink, J. Dankert, and S. A. J. Zaat. 2001. Mycoplasma pneumoniae P1 type 1- and type 2-specific sequences within the P1 cytadhesin gene of individual strains. Infect. Immun. 695612-5618. - PMC - PubMed
    1. Dumke, R., I. Catrein, E. Pirkl, R. Herrmann, and E. Jacobs. 2003. Subtyping of Mycoplasma pneumoniae isolates based on extended genome sequencing and on expression profiles. Int. J. Med. Microbiol. 292513-525. - PubMed

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