doi: 10.1016/0005-2744(77)90079-1.
Purification of methylmalonyl-CoA mutase from Propionibacterium shermanii using affinity chromatography
- PMID: 19074
- DOI: 10.1016/0005-2744(77)90079-1
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Purification of methylmalonyl-CoA mutase from Propionibacterium shermanii using affinity chromatography
Biochim Biophys Acta.
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Abstract
A novel procedure for the purification of methylmalonyl-CA mutase from Propionibacterium shermanii has been described which employs affinity chromatography on a column of immobilized vitamin B-12 linked covalently to Sepharose. The method has the advantage of being simple and rapid, thus enabling the purification of the enzyme to near homogeneity with good yields.
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