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. 1977 Aug 11;483(2):487-91.
doi: 10.1016/0005-2744(77)90079-1.

Purification of methylmalonyl-CoA mutase from Propionibacterium shermanii using affinity chromatography

V V Murthy, E Jones, T W Cole Jr, J Johnson Jr

Purification of methylmalonyl-CoA mutase from Propionibacterium shermanii using affinity chromatography

V V Murthy et al. Biochim Biophys Acta. .

Abstract

A novel procedure for the purification of methylmalonyl-CA mutase from Propionibacterium shermanii has been described which employs affinity chromatography on a column of immobilized vitamin B-12 linked covalently to Sepharose. The method has the advantage of being simple and rapid, thus enabling the purification of the enzyme to near homogeneity with good yields.

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