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. 1991 Apr;194(1):223-9.
doi: 10.1016/0003-2697(91)90171-o.

Bioassay for siderophore utilization by Candida albicans

A A Minnick  1 , L E Eizember, J A McKee, E K Dolence, M J Miller
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Bioassay for siderophore utilization by Candida albicans

A A Minnick et al. Anal Biochem. 1991 Apr.

Abstract

A convenient plate assay which is sensitive to medium pH has been developed to evaluate potential siderophores of Candida albicans. Adding a siderophore to a filter paper disk on chemically defined Lee's agar (final pH 7.2) seeded with the test strain reversed the growth inhibitory effects of the supplemented (25-100 micrograms/ml) iron chelator ethylenediaminedi(o-hydroxyphenylacetic acid), to provide a zone of growth stimulation. This bioassay has been used to demonstrate the structure-activity relationships of ferrichrome and several water-soluble hydroxamate peptide building blocks of this natural siderophore. Of all compounds so evaluated, ferrichrome exhibited the best activity.

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