Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Jan;6(1):79-81.
doi: 10.1038/nmeth.1278. Epub 2008 Dec 14.

Infrared laser-mediated gene induction in targeted single cells in vivo

Yasuhiro Kamei  1 , Motoshi Suzuki, Kenjiro Watanabe, Kazuhiro Fujimori, Takashi Kawasaki, Tomonori Deguchi, Yoshihiro Yoneda, Takeshi Todo, Shin Takagi, Takashi Funatsu, Shunsuke Yuba
Affiliations

Infrared laser-mediated gene induction in targeted single cells in vivo

Yasuhiro Kamei et al. Nat Methods. 2009 Jan.

Abstract

We developed infrared laser-evoked gene operator (IR-LEGO), a microscope system optimized for heating cells without photochemical damage. Infrared irradiation causes reproducible temperature shifts of the in vitro microenvironment in a power-dependent manner. When applied to living Caenorhabditis elegans, IR-LEGO induced heat shock-mediated expression of transgenes in targeted single cells in a more efficient and less deleterious manner than a 440-nm dye laser and elicited physiologically relevant phenotypic responses.

PubMed Disclaimer

References

    1. Biophys J. 2002 Apr;82(4):2224-31 - PubMed
    1. Dev Biol. 2007 Apr 15;304(2):800-10 - PubMed
    1. Development. 2000 May;127(9):1953-60 - PubMed
    1. Nat Biotechnol. 1998 Sep;16(9):833-8 - PubMed
    1. Development. 1996 Oct;122(10):3117-31 - PubMed

Publication types

MeSH terms

Substances