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. 2008 Oct;72(5):440-3.

Analytical sensitivity of air samplers based on uniform point-source exposure to airborne porcine reproductive and respiratory syndrome virus and swine influenza virus

Joseph R Hermann  1 , Jeffrey J Zimmerman
Affiliations

Analytical sensitivity of air samplers based on uniform point-source exposure to airborne porcine reproductive and respiratory syndrome virus and swine influenza virus

Joseph R Hermann et al. Can J Vet Res. 2008 Oct.

Abstract
in English, French

Research and surveillance activities involving airborne pathogens rely on the capture and enumeration of pathogens suspended in aerosols. The objective of this study was to estimate the analytical sensitivity (detection threshold) of each of 4 air samplers for Porcine reproductive and respiratory syndrome virus (PRRSV) and swine influenza virus (SIV). In a 5-min sampling period under controlled conditions, the analytical sensitivity of the AGI-30 (Ace Glass, Vineland, New Jersey, USA), AGI-4 (Ace Glass), SKC BioSampler (SKC, Eighty Four, Pennsylvania, USA), and Midwest Micro-Tek sampler (Midwest Micro-Tek, Brookings, South Dakota, USA) was calculated at 1 x 10(1.1), 1 x 10(1.3), 1 X 10(1.1), and 1 x 10(1.2) median tissue culture infectious dose (TCID50) equivalents for PRRSV and 1 x 10(1.4), 1 x 10(1.1), 1 x 10(1.6), and 1 x 10(1.2) TCID50 equivalents for SIV [per 60 L (5-min sampling period)]. Despite marked differences in sampler design, no statistically significant difference in analytical sensitivity was detected between the samplers for collection of artificially produced aerosols containing cell-culture-propagated PRRSV or SIV.

Les activités de recherche et de surveillance impliquant les agents pathogènes transmis par voie aérienne se fient sur la capture et l’énumération de ces agents en suspension dans les aérosols. L’objectif de la présente étude était d’estimer la sensibilité analytique (seuil de détection) de chacun de 4 échantillonneurs d’air pour le virus du syndrome reproducteur et respiratoire porcin (PRRSV) et le virus de l’influenza porcin (SIV). Durant une période d’échantillonnage de 5 minutes en conditions contrôlées, la sensibilité analytique des échantillonneurs AGI-30 (Ace Glass, Vineland, New Jersey, USA), AGI-4 (Ace Glass), SKC BioSampler (SKC, Eighty Four, Pennsylvania, USA) et Midwest Micro-Tek (Midwest Micro-Tek, Brookings, South Dakota, USA), exprimée en équivalent de dose infectante médiane de culture de tissu (TCID50), a été calculée comme étant 1 × 101,1, 1 × 101,3, 1 × 101,1, et 1 × 101,2 pour le PRRSV et 1 × 101,4, 1 × 101,1, 1 × 101,6, et 1 × 101,2 pour le SIV [par volume de 60 L (période d’échantillonnage de 5 minutes)]. Malgré des différences majeures dans le design des échantillonneurs, aucune différence statistiquement significative dans la sensibilité analytique n’a été détectée entre les échantillonneurs pour la collecte d’aérosols artificiellement produits contenant du PRRSV ou du SIV propagés en culture cellulaire.

(Traduit par Docteur Serge Messier)

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Figures

Figure 1
Figure 1
Experimental apparatus for estimating the analytical sensitivity of air samplers. A — collison nebulizer (BGI, Waltham, Massachusetts, USA); B — canine surgical mask; C — impinger (AGI-30; Ace Glass, Vineland, New Jersey, USA).
Figure 2
Figure 2
Determination of analytical sensitivity across viruses for AGI-30 (– ·· –), AGI-4 (—), SKC BioSampler (····), and Midwest Micro-Tek (- - -) using linear regression analysis. RT-PCR — reverse transcriptase polymerase chain reaction.
Figure 3
Figure 3
Determination of analytical sensitivity across impingers for Porcine reproductive and respiratory syndrome virus (—) and swine influenza virus (– –) by linear regression analysis.
See this image and copyright information in PMC

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