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Comparative Study
. 2009 Mar;401(1-2):73-5.
doi: 10.1016/j.cca.2008.11.021. Epub 2008 Nov 30.

Distinctive autofluorescence of urine samples from individuals with bacteriuria compared with normals

Ayad G Anwer  1 , Perinchery M Sandeep, Ewa M Goldys, Subramanyam Vemulpad
Affiliations
Comparative Study

Distinctive autofluorescence of urine samples from individuals with bacteriuria compared with normals

Ayad G Anwer et al. Clin Chim Acta. 2009 Mar.

Abstract

Background: A variety of fluorophores are present in normal human urine. Alteration in the autofluorescence of urine could result from physiological or pathological changes.

Method: This study investigates the differences in the autofluorescence of 45 normal urine samples from 25 individuals with bacteriuria.

Results: Excitation at 290 nm showed good discrimination between these 2 groups. Principal Component Analysis (PCA) of the data revealed statistically significant differences between the fluorescence spectra for samples with bacteriuria as compared to the control group.

Conclusion: The findings indicate the potential of the fluorescence spectrum of urine to be developed as a simple and rapid diagnostic tool.

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