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. 2009 Mar;104(4):935-7.
doi: 10.1007/s00436-008-1304-x. Epub 2008 Dec 17.

A simple method for long-term storage of Acanthamoeba species

Diana Axelsson-Olsson  1 , Jenny Olofsson, Patrik Ellström, Jonas Waldenström, Björn Olsen
Affiliations

A simple method for long-term storage of Acanthamoeba species

Diana Axelsson-Olsson et al. Parasitol Res. 2009 Mar.

Abstract

We present a novel and simple technique for storing live Acanthamoeba for long periods of time. The amoebae are maintained at refrigerator temperatures in a peptone-yeast extract-glucose (PYG) medium normally used for cultivation. Using this method, we obtained survival rates of at least 4 years for Acanthamoeba polyphaga and 3 years for Acanthamoeba castellanii and Acanthamoeba rhysodes. Advantages of this storage method are: (1) it is quick and simple, (2) inexpensive, (3) does not require encystment before storage, (4) resuscitation of cysts can be achieved within a week of culture in PYG medium at 27 degrees C, and does not require co-culture with bacteria or any special equipment.

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