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. 2009 Feb;47(2):469-71.
doi: 10.1128/JCM.01915-08. Epub 2008 Dec 17.

Molecular diagnostic identification of Staphylococcus pseudintermedius

Jeanette Bannoehr  1 , Alessia Franco, Manuela Iurescia, Antonio Battisti, J Ross Fitzgerald
Affiliations

Molecular diagnostic identification of Staphylococcus pseudintermedius

Jeanette Bannoehr et al. J Clin Microbiol. 2009 Feb.

Abstract

We report the first diagnostic test for the identification of Staphylococcus pseudintermedius involving a simple PCR-restriction fragment length polymorphism approach. The method allows discrimination of S. pseudintermedius from the closely related members of the Staphylococcus intermedius group and other important staphylococcal pathogens of humans and dogs.

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Figures

FIG. 1.
FIG. 1.
Agarose gel electrophoresis of MboI restriction digest pta PCR products. Lane 1, 100-bp ladder; lane 2, S. pseudintermedius ED99; lane 3, S. pseudintermedius ED99, MboI digested; lane 4, S. pseudintermedius HH1; lane 5, S. pseudintermedius HH1, MboI digested; lane 6, S. pseudintermedius Can6; lane 7, S. pseudintermedius Can6, MboI digested; lane 8, S. pseudintermedius Can10; lane 9, S. pseudintermedius Can10, MboI digested; lane 10, S. pseudintermedius LMG22219; lane 11, S. pseudintermedius LMG22219, MboI digested; lane 12, S. pseudintermedius 3414; lane 13, S. pseudintermedius 3414, MboI digested; lane 14, S. delphini ATCC 49171; lane 15, S. delphini ATCC 49171, MboI digested; lane 16, S. delphini 9106; lane 17, S. delphini 9106, MboI digested; lane 18, S. delphini HT20030680; lane 19, S. delphini HT20030680, MboI digested; lane 20, S. intermedius NCTC11048; lane 21, S. intermedius NCTC11048, MboI digested; lane 22, S. intermedius AV8061; lane 23, S. intermedius AV8061, MboI digested; lane 24, S. aureus Newman; lane 25, S. aureus Newman, MboI digested; lane 26, S. aureus N315; lane 27, S. aureus N315, MboI digested; lane 28, 100-bp ladder.
See this image and copyright information in PMC

References

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