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Comment
. 2008 Dec 18;359(25):2729; author reply 2729-31.
doi: 10.1056/NEJMc082011.

Amyloid and transplanted islets

Michael R Rickels, Heather W Collins, Ali Naji
Comment

Amyloid and transplanted islets

Michael R Rickels et al. N Engl J Med. .
No abstract available

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Figures

Figure 1
Figure 1. Plasma Insulin, Amylin, and Molar Ratios between Insulin and Amylin in Four Insulin-Independent Recipients of Islet Transplantation and Four Control Subjects without Diabetes
Insulin and total amylin concentrations were measured by radioimmunoassay and enzyme-linked immunosorbent assay, respectively, in samples collected before and 2 to 5 minutes after the injection of 5 g of arginine under fasting conditions and hyperglycemic-clamp conditions with plasma glucose concentrations of 230 mg per deciliter and 340 mg per deciliter. Islet-transplant recipients had received a median of two islet infusions a median of 3 months before the study. Among islet-transplant recipients and control subjects, mean (±SE) values were similar for age (48±4 years vs. 43±3 years), body-mass index, defined as the weight in kilograms divided by square of the height in meters (20.4±1.1 vs. 23.2±1.4), and glycated hemoglobin (5.9±0.1% vs. 5.5±0.2%). The incremental insulin responses to arginine among islet-transplant recipients and control subjects were 107±19 pmol per liter versus 193±46 pmol per liter under fasting conditions (P not significant), 209±26 pmol per liter versus 1028±208 pmol per liter with a plasma glucose concentration of 230 mg per deciliter (P<0.01), and 296±54 pmol per liter versus 1322±379 pmol per liter with a plasma glucose concentration of 340 mg per deciliter (P<0.05). The corresponding amylin responses among islet-transplant recipients and control subjects under the three conditions were 2.8±0.8 pmol per liter versus 7.3±2.9 pmol per liter (P not significant), 8.7±1.7 pmol per liter versus 8.4±1.5 pmol per liter (P not significant), and 8.5±1.2 pmol per liter versus 21.3±3.6 pmol per liter (P<0.05). The corresponding secretory ratios of insulin to amylin were 14.4±7.5 versus 13.4±8.3 (P not significant), 1.9±5.3 versus 27.2±4.9 (P<0.05), and 8.2±2.8 versus 24.5±5.4 (P<0.05). Comparisons between groups were performed with the use of two-tailed unpaired Student's t-tests.
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References

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