Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Mar;53(3):1268-70.
doi: 10.1128/AAC.01300-08. Epub 2008 Dec 22.

Changes in aac(6')-Ib-cr prevalence and fluoroquinolone resistance in nosocomial isolates of Escherichia coli collected from 1991 through 2005

Gabriela Warburg  1 , Maya Korem, Ari Robicsek, Dalia Engelstein, Allon E Moses, Colin Block, Jacob Strahilevitz
Affiliations

Changes in aac(6')-Ib-cr prevalence and fluoroquinolone resistance in nosocomial isolates of Escherichia coli collected from 1991 through 2005

Gabriela Warburg et al. Antimicrob Agents Chemother. 2009 Mar.

Abstract

Clinical isolates of Escherichia coli collected from 1991 through 2005 at a tertiary-care center were studied for qnr and aac(6')-Ib-cr genes. Isolates bearing aac(6')-Ib-cr emerged in 1998, coinciding with an increase in ciprofloxacin resistance. The presence of aac(6')-Ib-cr was multiclonal and was associated with the presence of extended-spectrum beta-lactamases.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Polyacrylamide gel analysis of gap-LCR assay. Lane 1, low-molecular-weight DNA ladder (New England Biolabs, Beverly, MA); lane 2, pBAD24-aac(6′)-Ib-cr; lane 3, pBAD24-aac(6′)-Ib; lane 4, aac(6′)-Ib-cr-negative E. coli; lane 5, aac(6′)-Ib-cr-positive E. coli. Arrow points to the putative reaction product.
FIG. 2.
FIG. 2.
Percentage of clinical E. coli isolates (n = 718) harboring resistance to ciprofloxacin (MIC ≥ 2 μg/ml) and harboring aac(6′)-Ib-cr. No aac(6′)-Ib-cr genes were found before 1998.
FIG. 3.
FIG. 3.
PFGE of 10 E. coli isolates from 2004 and E. coli KL-16 (serving as the control strain) after restriction with XbaI. Lane 1, phage lambda ladder (size marks in Kb); lanes 2 and 12, E. coli KL-16; lanes 3 to 11, E. coli isolates from 2004 (in lane 4, one of the five PFGE-identical isolates).
See this image and copyright information in PMC

References

    1. Abravaya, K., J. J. Carrino, S. Muldoon, and H. H. Lee. 1995. Detection of point mutations with a modified ligase chain reaction (Gap-LCR). Nucleic Acids Res. 23:675-682. - PMC - PubMed
    1. Ambrozic Avgustin, J., R. Keber, K. Zerjavic, T. Orazem, and M. Grabnar. 2007. Emergence of the quinolone resistance-mediating gene aac(6′)-ib-cr in extended-spectrum-β-lactamase-producing Klebsiella isolates collected in Slovenia between 2000 and 2005. Antimicrob. Agents Chemother. 51:4171-4173. - PMC - PubMed
    1. Clinical and Laboratory Standards Institute. 2007. Performance standards for antimicrobial susceptibility testing; 15th informational supplement. M100-S17. Clinical and Laboratory Standards Institute, Wayne, PA.
    1. Coque, T. M., A. Novais, A. Carattoli, L. Poirel, J. Pitout, L. Peixe, F. Baquero, R. Canton, and P. Nordmann. 2008. Dissemination of clonally related Escherichia coli strains expressing extended-spectrum beta-lactamase CTX-M-15. Emerg. Infect. Dis. 14:195-200. - PMC - PubMed
    1. Endimiani, A., L. L. Carias, A. M. Hujer, C. R. Bethel, K. M. Hujer, F. Perez, R. A. Hutton, W. R. Fox, G. S. Hall, M. R. Jacobs, D. L. Paterson, L. B. Rice, S. G. Jenkins, F. C. Tenover, and R. A. Bonomo. 2008. Presence of plasmid-mediated quinolone resistance in Klebsiella pneumoniae isolates possessing blaKPC in the United States. Antimicrob. Agents Chemother. 52:2680-2682. - PMC - PubMed

Publication types

MeSH terms