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. 2009 Jan;9(2):434-50.
doi: 10.1002/pmic.200800477.

A comprehensive analysis of Trypanosoma brucei mitochondrial proteome

Aswini K Panigrahi  1 , Yuko Ogata, Alena Zíková, Atashi Anupama, Rachel A Dalley, Nathalie Acestor, Peter J Myler, Kenneth D Stuart
Affiliations

A comprehensive analysis of Trypanosoma brucei mitochondrial proteome

Aswini K Panigrahi et al. Proteomics. 2009 Jan.

Abstract

The composition of the large, single, mitochondrion (mt) of Trypanosoma brucei was characterized by MS (2-D LC-MS/MS and gel-LC-MS/MS) analyses. A total of 2897 proteins representing a substantial proportion of procyclic form cellular proteome were identified, which confirmed the validity of the vast majority of gene predictions. The data also showed that the genes annotated as hypothetical (species specific) were overpredicted and that virtually all genes annotated as hypothetical, unlikely are not expressed. By comparing the MS data with genome sequence, 40 genes were identified that were not previously predicted. The data are placed in a publicly available web-based database (www.TrypsProteome.org). The total mitochondrial proteome is estimated at 1008 proteins, with 401, 196, and 283 assigned to the mt with high, moderate, and lower confidence, respectively. The remaining mitochondrial proteins were estimated by statistical methods although individual assignments could not be made. The identified proteins have predicted roles in macromolecular, metabolic, energy generating, and transport processes providing a comprehensive profile of the protein content and function of the T. brucei mt.

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Figures

Figure 1
Figure 1
Summary of protein identification from cellular and sub-cellular fractions. Number of proteins identified in detergent soluble (supernatant) and insoluble fractions (pellet) from whole cell and mitochondrial enriched fraction and comparison between them are shown.
Figure 2
Figure 2
Comparison of proportions of protein groups annotated in GeneDB database to that identified by proteomics. Percent of proteins with known or assigned function are denoted by filled line, hypothetical conserved by gray, hypothetical (species specific) by black, and hypothetical, unlikely by white areas in the pie-chart.
Figure 3
Figure 3
Assessing the ability of available software for prediction of mitochondrial proteins in T. brucei. Schematics showing representative scores obtained for presence of mitochondrial import signal in a group of (A) known mitochondrial proteins and (B) non-mitochondrial proteins using Mitoprot (open bar) and SignalP (gray bar) programs.
See this image and copyright information in PMC

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