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. 2009 Jan;15(1):87-90.
doi: 10.3201/eid1501.080427.

Serotype g12 rotaviruses, Lilongwe, Malawi

Nigel A Cunliffe  1 , Bagrey M Ngwira, Winifred Dove, Osamu Nakagomi, Toyoko Nakagomi, Arantza Perez, C Anthony Hart, Peter N Kazembe, Charles C V Mwansambo
Affiliations

Serotype g12 rotaviruses, Lilongwe, Malawi

Nigel A Cunliffe et al. Emerg Infect Dis. 2009 Jan.

Abstract

To assess diversity of rotavirus strains in Lilongwe, Malawi, we conducted a cross-sectional study of children with acute gastroenteritis, July 2005-June 2007. Serotype G12 was identified in 30 (5%) of 546 rotavirus-positive fecal specimens. The G12 strain possessed multiple electropherotypes and P-types, but their viral protein 7 sequences were closely related, indicating that reassortment has occurred.

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Figures

Figure 1
Figure 1
Polyacrylamide gel electrophoresis and silver staining of rotavirus double-stranded RNA of representative serotype G12 strains from Lilongwe, Malawi. RNA segments are indicated to the left. Strains Wa (long electropherotype) and KUN (short electropherotype) are controls. Field strains, designated electropherotype profiles, and P types are as follows: Lane 1, KCH958 short – profile S1, P[6]; lane 2, KCH1120, long – profile L1, P[6]; lane 3, KCH1124, short – profile S1, P[6]; lane 4, KCH1050, short – profile S1, P[6]; lane 5, LOP286, long – profile L2, P[6]; lane 6, LOP523, short – profile S1, P[6]; lane 7, KCH944, short – profile S1, P[6]; lane 8, KCH1074, short – profile S2, P[6]; lane 9, KCH569, long – profile L2, P[6]; lane 10, KCH602, long – profile L2, P[6]; lane 11, KCH344, long – profile L3, P[8].
Figure 2
Figure 2
Phylogenetic tree based on VP7 nucleotide sequences from representative Malawi G12 strains and G12 strains deposited in DNA databases. Strain designations are followed by country of origin. Malawi strain KCH1051 is genotype G12P[8] and has an indeterminate RNA profile. Horizontal lengths are proportional to the genetic distance calculated with the Kimura 2-parameter method. Number adjacent to the node represents the bootstrap value of 1,000 replicates and values <80% are not indicated. Scale bar shows genetic distance expressed as nucleotide substitutions per site.
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