Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2008 Nov;8(4):381-5.
doi: 10.17305/bjbms.2008.2904.

In vitro examination of ontogenesis of developing neuronal cells in vagal nuclei in medulla oblongata in newborns

Hilmi Islami  1 , Ragip Shabani, Sadi Bexheti, Ibrahim Behluli, Aziz Sukalo, Denis Raka, Rozafa Koliqi, Naim Haliti, Hilmi Dauti, Shaip Krasniqi, Mentor Disha
Affiliations

In vitro examination of ontogenesis of developing neuronal cells in vagal nuclei in medulla oblongata in newborns

Hilmi Islami et al. Bosn J Basic Med Sci. 2008 Nov.

Abstract

The development of neuron cells in vagal nerve nuclei in medulla oblongata was studied in vitro in live newborns and stillborns from different cases. Morphological changes were studied in respiratory nuclei of dorsal motor centre (DMNV) and nucleus tractus solitarius (NTS) in medulla oblongata. The material from medulla oblongata was fixated in 10 micro buffered formalin solution. Fixated material was cut in series of 10mu thickness, with starting point from obex in +/- 4 mm thickness. Special histochemical and histoenzymatic methods for central nervous system were used: cresyl echt violet coloring, tolyidin blue, Sevier-Munger modification and Grimelius coloring. In immature newborns (abortions and immature) in dorsal motor nucleus of the vagus (DMNV) population stages S1, S2, S3 are dominant. In neuron population in vagal sensory nuclei (NTS) stages S1, S2 are dominant. In more advanced stages of development of newborns (premature), in DMNV stages S3 and S4 are seen and in NTS stages S2 and S3 are dominant. In mature phase of newborns (maturity) in vagal nucleus DMNV stages S5 and S6 are dominant, while in sensory nucleus NTS stages S4 and S5 are dominant. These data suggest that neuron population in dorsal motor nucleus of the vagus (DMNV) are more advanced in neuronal maturity in comparison with sensory neuron population of vagal sensory nucleus NTS. This occurrence shows that phylogenetic development of motor complex is more advanced than the sensory one, which is expected to take new information's from the extra uterine life after birth (extra uterine vagal phenotype).

PubMed Disclaimer

Figures

FIGURE 1
FIGURE 1
Horizontal serial cut in medulla oblongata, starting from obex. Population of neurons in nucleus DMNV and NTS in newborns; (H&E; 100 x).
FIGURE 2
FIGURE 2
Horizontal serial exit in obex level (arrow) in abortus. Migratory alignment of new neuroblasts is seen; (H&E; 400 x).
FIGURE 3
FIGURE 3
Population of neurons in stages SI andS2 in nucleus DMNVin newborns; (Cresyl violet 400 x).
FIGURE 4
FIGURE 4
Population of neurons in nucleus DMNV stages SI, S2, S3 of development in newborns; (PTAH; 400 x).
FIGURE 5
FIGURE 5
Stage S4 of neuroblasts in vagal nudei in newborns; (LuxolfestbluMBS;400x).
FIGURE 6
FIGURE 6
Stages S5 and S6 of cell development in matures; (PTAH;400x).
FIGURE 7
FIGURE 7
Pronounced cholinoreactivity in neuron populations in nucleus of NTS in medulla oblongata, in matures; (Indoxyl HAT and ACHE; 400 x).
FIGURE 8
FIGURE 8
Migration ofcholinoreacüve neuroblasts along blood vessel in neuron population level in DMNV in newborns; (Indoxyl HAT and ACHE; 400 x).
See this image and copyright information in PMC

References

    1. Erlichmana JS. Nicotinic receptors mediate spontaneous GABA release in the rat dorsal motor nucleus of the vagus. Neuroscience Aug. 1997;79:671–681. - PubMed
    1. Goodwin BP, Anderson GF, Barraco RA. Characterization of muscarinic receptors in the rat NTS. Neurosci Lett May. 1995;19:131–135. - PubMed
    1. Miguel MP. Neuropathologic correlates of perinatal asphyxia. Int. Pediatr. 2000;15(4):221–228.
    1. Kenney HC, Armstrong DD. Perinatal Neuropathology. In: Graham DI, Lantos PL, editors. Greenfield’s Neuropathology. London: Arnold Publushers; 1997.
    1. Fitzgerald JT. Basic and Applied. Tindal. Vol. 122. London: 1985. Neuroanatomy; pp. 146–148.