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. 2009 Mar;47(3):870-1.
doi: 10.1128/JCM.02306-08. Epub 2009 Jan 7.

Susceptibility of Vibrio parahaemolyticus to tris-dependent DNA degradation during pulsed-field gel electrophoresis

Swapan K Banerjee, Jeffrey M Farber

Susceptibility of Vibrio parahaemolyticus to tris-dependent DNA degradation during pulsed-field gel electrophoresis

Swapan K Banerjee et al. J Clin Microbiol. 2009 Mar.
No abstract available

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Figures

FIG. 1.
FIG. 1.
PFGE patterns of V. parahaemolyticus strains digested with NotI and run with regular TBE buffer (A) and TBE buffer to which thiourea was added (B). Four seafood isolates and two standard strains (Table 1) were processed without (lanes 1 to 6) as well as with (lanes 7 to 12) formaldehyde fixation and run separately with the two buffers. Lanes M, standard DNA size markers, consisting of DNA from Salmonella enterica serovar Braenderup H9812 digested with 50 U of XbaI at 37°C for 2 h.
See this image and copyright information in PMC

References

    1. Banerjee, S. K., S. Pandian, E. C. Todd, and J. M. Farber. 2002. A rapid and improved method for the detection of Vibrio parahaemolyticus and Vibrio vulnificus strains grown on hydrophobic grid membrane filters. J. Food Prot. 651049-1053. - PubMed
    1. Centers for Disease Control and Prevention. 2002. Standardized molecular subtyping of foodborne bacterial pathogens by pulsed-field gel electrophoresis. Centers for Disease Control and Prevention, Atlanta, GA.
    1. Inglis, T. J. J., L. O'Reilly, N. Foster, A. Clair, and J. Sampson. 2002. Comparison of rapid, automated ribotyping and DNA macrorestriction analysis of Burkholderia pseudomallei. J. Clin. Microbiol. 403198-3203. - PMC - PubMed
    1. Leclair, D., F. Pagotto, J. M. Farber, B. Cadieux, and J. Austin. 2006. Comparison of DNA fingerprinting methods for use in investigation of type E botulism outbreaks in the Canadian Arctic. J. Clin. Microbiol. 441635-1644. - PMC - PubMed
    1. Marshall, S., C. G. Clark, G. Wang, M. Mulvey, M. T. Kelly, and W. M. Johnson. 1999. Comparison of molecular methods for typing Vibrio parahaemolyticus. J. Clin. Microbiol. 372473-2478. - PMC - PubMed

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