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. 2009 Jan;96(1):L01-3.
doi: 10.1016/j.bpj.2008.09.032.

In vivo labeling method using a genetic construct for nanoscale resolution microscopy

Jan Schröder  1 , Hélène Benink, Marcus Dyba, Georgyi V Los
Affiliations

In vivo labeling method using a genetic construct for nanoscale resolution microscopy

Jan Schröder et al. Biophys J. 2009 Jan.

Abstract

We demonstrate beam scanning-stimulated emission depletion microscopy with in vivo labeled cells. A red emitting fluorescent dye is introduced into membrane protein fused to a multifunctional reporter protein (HaloTag, Promega, Madison, WI) in live cells. This approach allows superresolution stimulated emission depletion imaging without the limitations of immunofluorescence-based staining.

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Figures

Figure 1
Figure 1
STED image showing an in vivo labeled HeLa cell (A). Detailed areas (B) showing filopodia imaged in STED mode (left) or confocal mode (right). Each region is 2-μm-square size. Profiles in 1 and 2 are referring to Fig. 2.
Figure 2
Figure 2
Showing the intensity profiles of Fig. 1B. (A) Thickness of one filopodium in detailed area 2. (B) Distance between to adjacent filopodia in detailed area 1.
See this image and copyright information in PMC

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