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. 2009 Feb 15;19(4):1210-3.
doi: 10.1016/j.bmcl.2008.12.071. Epub 2008 Dec 25.

Synthesis and evaluation of a cyclic imine derivative conjugated to a fluorescent molecule for labeling of proteins

Hai-Ming Guo  1 , Maki Minakawa, Lynn Ueno, Fujie Tanaka
Affiliations

Synthesis and evaluation of a cyclic imine derivative conjugated to a fluorescent molecule for labeling of proteins

Hai-Ming Guo et al. Bioorg Med Chem Lett. .

Abstract

A cyclic imine conjugated to a fluorescent dansyl group was synthesized and used for covalent labeling of proteins. The covalent attachment to proteins was confirmed by gel electrophoresis and mass analysis.

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Figures

Figure 1
Figure 1
Analysis of proteins modified with 8 after separation by gel electrophoresis. Lane a, protein standard marker; b, lysozyme; c, chymotrypsinogen (see text); d, myoglobin; e, carbonic anhydrase; f, cytochrome C. (A) Analysis of fluorescence of dansyl group attached to proteins under UV 312 nm. (B) Analysis of proteins by Coomassie staining.
Scheme 1
Scheme 1
Scheme 2
Scheme 2
Scheme 3
Scheme 3
See this image and copyright information in PMC

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