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. 2009 Apr;116(4):487-91.
doi: 10.1007/s00702-008-0143-3. Epub 2009 Jan 13.

Expression of four housekeeping proteins in elderly patients with schizophrenia

Deborah Elaine Bauer  1 , Vahram Haroutunian, Robert E McCullumsmith, James H Meador-Woodruff
Affiliations

Expression of four housekeeping proteins in elderly patients with schizophrenia

Deborah Elaine Bauer et al. J Neural Transm (Vienna). 2009 Apr.

Abstract

We compared protein expression by Western blot analysis in four areas of postmortem brain from patients with schizophrenia and control subjects for several proteins that are often used as controls for Western blot studies: beta-tubulin, actin, glyceraldehyde-3-phosphate dehydrogenase, and valosin-containing protein. We did not detect any differences in expression between subjects with schizophrenia and a comparison group. These results suggest that all four proteins are suitable loading controls for postmortem studies of schizophrenia.

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Figures

Fig. 1
Fig. 1
Housekeeping protein expression in multiple brain regions. Data are expressed as ratio of signal intensity for subjects with schizophrenia divided by signal intensity for comparison subjects. None of these proteins differed between diagnostic groups in any region studied. On the right are representative Western blots for the four proteins studied. ACC anterior cingulate cortex, DLPFC dorsolateral prefrontal cortex, HPC hippocampus, PVC primary visual cortex, C comparison subject, S schizophrenia, VCP valosin-containing protein, GAPDH glyceraldehyde-3-phosphate dehydrogenase, kDa kilodaltons
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References

    1. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248–254. - PubMed
    1. Eastwood SL, Harrison PJ. Synaptic pathology in the anterior cingulate cortex in schizophrenia and mood disorders. A review and a Western blot study of synaptophysin, GAP-43 and the complexins. Brain Res Bull. 2001;55(5):569–578. - PubMed
    1. Ferguson RE, Carroll HP, Harris A, Maher ER, Selby PJ, Banks RE. Housekeeping proteins: a preliminary study illustrating some limitations as useful references in protein expression studies. Proteomics. 2005;5(2):566–571. - PubMed
    1. Liu NK, Xu XM. Beta-tubulin is a more suitable internal control than beta-actin in western blot analysis of spinal cord tissues after traumatic injury. J Neurotrauma. 2006;23(12):1794–1801. - PubMed
    1. McNulty SE, Toscano WA., Jr Transcriptional regulation of glyceraldehyde-3-phosphate dehydrogenase by 2, 3, 7, 8-tetra-chlorodibenzo-p-dioxin. Biochem Biophys Res Commun. 1995;212(1):165–171. - PubMed