Relevance of in vitro neurotoxicity testing for regulatory requirements: challenges to be considered
- PMID: 19150401
- DOI: 10.1016/j.ntt.2008.12.003
Relevance of in vitro neurotoxicity testing for regulatory requirements: challenges to be considered
Abstract
The current testing requirements for both adult and developmental neurotoxicity evaluation are based on in vivo animal models and the neurotoxic potency of compounds is mainly determined by neurobehavioural and neuropathological effects. In vitro studies are considered complementary to animal tests because they provide an understanding of the molecular/cellular mechanisms involved in neurotoxicity. However, the selection of relevant in vitro neuronal/glial specific endpoints applied to various neuronal cellular models should be done in a careful way to build reliable and feasible testing strategies since usually these endpoints have to be tested in various complementary in vitro systems. The requirements for applying a more complex test strategy where toxicokinetic aspects are included together with different tools to compensate for the lack of in vitro metabolic competence are discussed. Taking into consideration the recent European Commission chemical legislation concerning registration, evaluation and authorisation of chemicals (REACH) it has become a priority to develop new intelligent testing strategies integrating computational models and in vitro assays based on cell culture models and endpoints that are amenable for adaptation to high throughput screening to be able to test a large number of chemicals.
Copyright 2009 Elsevier Inc. All rights reserved.
Similar articles
-
Strategies and tools for preventing neurotoxicity: to test, to predict and how to do it.Neurotoxicology. 2012 Aug;33(4):796-804. doi: 10.1016/j.neuro.2012.01.019. Epub 2012 Feb 7. Neurotoxicology. 2012. PMID: 22342764
-
Neural progenitor cells as models for high-throughput screens of developmental neurotoxicity: state of the science.Neurotoxicol Teratol. 2010 Jan-Feb;32(1):4-15. doi: 10.1016/j.ntt.2009.06.005. Epub 2009 Jun 24. Neurotoxicol Teratol. 2010. PMID: 19559083 Review.
-
In vitro developmental neurotoxicity (DNT) testing: relevant models and endpoints.Neurotoxicology. 2010 Sep;31(5):545-54. doi: 10.1016/j.neuro.2009.11.006. Epub 2009 Dec 5. Neurotoxicology. 2010. PMID: 19969020
-
Safety and nutritional assessment of GM plants and derived food and feed: the role of animal feeding trials.Food Chem Toxicol. 2008 Mar;46 Suppl 1:S2-70. doi: 10.1016/j.fct.2008.02.008. Epub 2008 Feb 13. Food Chem Toxicol. 2008. PMID: 18328408 Review.
-
Use of the dog as non-rodent test species in the safety testing schedule associated with the registration of crop and plant protection products (pesticides): present status.Arch Toxicol. 2005 Nov;79(11):615-26. doi: 10.1007/s00204-005-0678-0. Epub 2005 Jun 7. Arch Toxicol. 2005. PMID: 15940470
Cited by
-
Developmental neurotoxicity - challenges in the 21st century and in vitro opportunities.ALTEX. 2014;31(2):129-56. doi: 10.14573/altex.1403271. ALTEX. 2014. PMID: 24687333 Free PMC article. Review.
-
Misadventures in Toxicology: Concentration Matters for Testosterone-Induced Neurotoxicity.Toxics. 2023 Mar 10;11(3):258. doi: 10.3390/toxics11030258. Toxics. 2023. PMID: 36977023 Free PMC article.
-
Integrated Approach for Testing and Assessment for Developmental Neurotoxicity (DNT) to Prioritize Aromatic Organophosphorus Flame Retardants.Toxics. 2024 Jun 18;12(6):437. doi: 10.3390/toxics12060437. Toxics. 2024. PMID: 38922117 Free PMC article.
-
State-of-the-art of 3D cultures (organs-on-a-chip) in safety testing and pathophysiology.ALTEX. 2014;31(4):441-77. doi: 10.14573/altex.1406111. Epub 2014 Jul 14. ALTEX. 2014. PMID: 25027500 Free PMC article. Review.
-
Evaluation of the neurotoxic/neuroprotective role of organoselenides using differentiated human neuroblastoma SH-SY5Y cell line challenged with 6-hydroxydopamine.Neurotox Res. 2012 Aug;22(2):138-49. doi: 10.1007/s12640-012-9311-1. Epub 2012 Jan 20. Neurotox Res. 2012. PMID: 22271527
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources