doi: 10.1007/978-1-60327-567-5_8.
Reflectometric interference spectroscopy
Affiliations
- PMID: 19151940
- DOI: 10.1007/978-1-60327-567-5_8
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Reflectometric interference spectroscopy
Methods Mol Biol.
2009.
Abstract
Reflectometry is classified in comparison to the commercialized refractometric surface plasmon resonance (SPR). The advantages of direct optical detection depend on a sophisticated surface chemistry resulting in negligible nonspecific binding and high loading with recognition sites at the biopolymer sensitive layer of the transducer. Elaborate details on instrumental realization and surface chemistry are discussed for optimum application of reflectometric interference spectroscopy (RIfS). A standard protocol for a binding inhibition assay is given. It overcomes principal problems of any direct optical detection technique.
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