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Review
. 2009;49(1):157-66.
doi: 10.1016/j.advenzreg.2008.12.003. Epub 2008 Dec 31.

Nuclear lamins and chromatin: when structure meets function

Thomas Dechat  1 , Stephen A Adam, Robert D Goldman
Affiliations
Review

Nuclear lamins and chromatin: when structure meets function

Thomas Dechat et al. Adv Enzyme Regul. 2009.
No abstract available

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Figures

Figure 1
Figure 1
Structure of nuclear lamins. Schematic drawing of a lamin polypeptide chain depicting the α-helical central rod domain, the N-terminal globular head domain and the C-terminal globular tail domain. In addition the nuclear localization signal (NLS) and the Ig-fold are indicated.
Figure 2
Figure 2
Close association of heterochromatin with the nuclear lamina. Localization of lamins A/C (C, G), histone H3 trimethylated (H3me3) on lysine 9 (H3K9me3) (B), or on lysine 27 (H3K27me3) (F) in human foreskin fibroblasts (A–D) and human dermal fibroblasts from a female donor (E–H). DNA is stained with Hoechst dye (blue; A, E). Note that long stretches of heterochromatin, as revealed by Hoechst staining and by staining for H3K9me3, a histone modification associated mainly with constitutive pericentric heterochromatin, are in close proximity to and partially overlapping with peripheral lamins A/C in human foreskin fibroblasts (A–D). In addition, the Xi, which represents a large heterochromatic mass that can be visualized by staining with Hoechst and H3K27me3 (see arrowheads), is often found associated with lamins at the nuclear lamina (E–H). Scale bars, 10 μM.
Figure 3
Figure 3
Lamin B3 is closely associated with PCNA and chromatin in in vitro assembled nuclei. Localization of Xenopus lamin B3 (B, b; green) and PCNA (C, c; red) in a nucleus assembled in a Xenopus egg interphase extract for 130 min (A–D). DNA is stained with Hoechst dye (A, a; blue). The area in the box in D is enlarged (3.5×) to show the partial overlap between DNA, lamin B3 and PCNA (a–d). Brightness and contrast are enhanced in b compared to B for better visualization of the internal lamin B3 structures. Scale bar, 5 μM.
See this image and copyright information in PMC

References

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