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. 2009 Feb;44(2):115-8.
doi: 10.1016/j.jcv.2008.12.004. Epub 2009 Jan 20.

Detection of WU polyomavirus DNA by real-time PCR in nasopharyngeal aspirates, serum, and stool samples

Florian Neske  1 , Kerstin Blessing, Anika Pröttel, Franziska Ullrich, Hans Wolfgang Kreth, Benedikt Weissbrich
Affiliations

Detection of WU polyomavirus DNA by real-time PCR in nasopharyngeal aspirates, serum, and stool samples

Florian Neske et al. J Clin Virol. 2009 Feb.

Abstract

Background: The human WU polyomavirus (WUPyV) has been recently described as a novel virus in respiratory tract samples.

Objective: To investigate the viral load of WUPyV in nasopharyngeal aspirates (NPAs), stool, and serum samples of pediatric patients with acute respiratory tract diseases.

Study design: We established a real-time PCR for WUPyV DNA and tested NPA obtained between 2002 and 2007 from pediatric in-patients with acute respiratory tract diseases. In addition, 14 stool and 14 serum samples of children with WUPyV DNA positive NPA were analysed.

Results: WUPyV DNA was found in 5.2% of 1232 NPA. The median viral load in the NPA was 950 copies/ml (maximum 3.4 x 10(10) copies/ml). The WUPyV load in NPA was neither associated with the coinfection status nor with the clinical diagnoses. WUPyV DNA was found in 3 of 14 serum samples and in 2 of 14 stool samples. The WUPyV load in NPA tended to be higher in viremic children.

Conclusion: WUPyV DNA was found in NPA, serum, and stool of hospitalised children with acute respiratory tract diseases. Further studies are necessary to determine whether WUPyV is a human pathogen.

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Figures

Fig. 1
Fig. 1
Viral load of WUPyV in NPA according to the clinical diagnosis. The median values (horizontal bars) were 9.3 × 102 copies/ml for all NPA, 2.5 × 103 copies/ml for upper respiratory tract disease (URTD), 3.0 × 103 copies/ml for pneumonia, 9.3 × 102 copies/ml for bronchitis, 6.8 × 102 copies/ml for wheezing bronchitis, and 9.3 × 102 copies/ml for febrile seizure.
Fig. 2
Fig. 2
WUPyV load in NPA of children with and without respiratory coinfections. In addition to WUPyV DNA, all samples were tested for antigen of RSV, influenza A/B virus, adenovirus, and parainfluenza viruses 1–3 by immunofluorescence assay and for hBoV DNA by PCR. Coinfecting agents found more than five times are displayed. Horizontal bars indicate median values.
Fig. 3
Fig. 3
Comparison of WUPyV loads in NPA according to WUPyV status of stool and serum samples of the same patient. Median values are indicated by horizontal bars.
See this image and copyright information in PMC

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