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Comparative Study
. 2009 Apr;47(4):1101-6.
doi: 10.1128/JCM.01963-08. Epub 2009 Jan 21.

First evaluation of the WASP, a new automated microbiology plating instrument

Paul P Bourbeau  1 , Brandi Lynn Swartz
Affiliations
Comparative Study

First evaluation of the WASP, a new automated microbiology plating instrument

Paul P Bourbeau et al. J Clin Microbiol. 2009 Apr.

Abstract

Many laboratories are experiencing growing shortages of trained microbiology technologists and technicians. Consequently, there is considerable interest in new automation that could potentially lessen labor demands for specimen processing. In this study, we present the first published evaluation of a new microbiology instrument, the Walk Away Specimen Processor (WASP), manufactured by Copan, Inc., in which we evaluated cross-contamination, the accuracy of plating, and the quality of the results. The absence of cross-contamination was demonstrated by plating a total of 200 alternating inoculated and sterile specimen tubes. The ability of the WASP to subculture enrichment broths was evaluated with 106 Lim broth specimens, with the results being identical to those obtained by testing by routine methods. Plating of urine specimens with the WASP was compared to plating with the Dynacon Inoculab instrument. Three hundred specimens were plated in duplicate on both instruments with 1-microl loops, and 293 specimens were plated in duplicate on both instruments with 10-microl loops. The results of duplicate plating with the same instrument (replicate plating) and of the consensus agreement between the two instruments were compared. The replicate plating results were comparable for both instruments, while the WASP had more specimens with significant results than the Inoculab with the 1-microl loop only. Lastly, for the plating of 113 specimens in ESwab tubes, the manual method and WASP plating each yielded 90 potential pathogens. In summary, we report the first evaluation of a new microbiology specimen-plating instrument, the WASP, which offers opportunities for the automated plating of microbiology specimens to an extent that has not been possible to date.

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Figures

FIG. 1.
FIG. 1.
Front view of the WASP. The dimensions of the instrument are 75 in. wide, 75 in. high, and 43 in. deep.
FIG. 2.
FIG. 2.
Top view of the WASP. The two SCARA robots are referred to as Tarzan and Jane.
FIG. 3.
FIG. 3.
Triquetra loops: from left to right, 10-μl, 30-μl, and 1-μl loops.
See this image and copyright information in PMC

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