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. 2009 Mar;47(3):774-6.
doi: 10.1128/JCM.02374-08. Epub 2009 Jan 21.

Performance of microcalorimetry for early detection of methicillin resistance in clinical isolates of Staphylococcus aureus

Daniela Baldoni  1 , Heinz Hermann, Reno Frei, Andrej Trampuz, Andrea Steinhuber
Affiliations

Performance of microcalorimetry for early detection of methicillin resistance in clinical isolates of Staphylococcus aureus

Daniela Baldoni et al. J Clin Microbiol. 2009 Mar.

Abstract

We describe a calorimetric assay for the detection of methicillin-resistant Staphylococcus aureus (MRSA) within 5 h. Microbial heat was calculated in culture with and without cefoxitin. Among 30 genetically distinct clinical isolates, 19/20 MRSA (95%) and 10/10 methicillin-susceptible Staphylococcus aureus (100%) were correctly identified. Microcalorimetry may be useful for rapid MRSA screening.

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Figures

FIG. 1.
FIG. 1.
Heat-flow (A) and total heat (B) of both reference MSSA ATCC 29213 and MRSA COL strains cultured in the presence of cefoxitin at 4 μg/ml (solid lines) or without antibiotic (dashed lines).
FIG. 2.
FIG. 2.
Relative heat distribution in 20 repeated measurements of the reference strains MSSA ATCC 29213 and MRSA COL (A); and 30 clinical isolates, 10 different MSSA and 20 different MRSA strains (B). Relative heat is calculated as the ratio between the total heat in the presence and absence of 4 μg/ml cefoxitin after 3 h, 4 h, and 5 h of incubation. Open circles indicate MSSA and closed circles MRSA; the horizontal line indicates the cutoff value of relative heat (0.4) used for the discrimination of MSSA and MRSA.
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